Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene disruption
|
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 28851956 |
MR4 number |
|
top of page |
Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
RMgm-928
|
Other information parent line | P.berghei ANKA 1804cl1 (RMgm-928) is a reference line expressing mCherry under the control of the P. berghei hsp70 promoter. The mutant does not contain a drug-selectable marker. The mCherry expression cassette has been introduced into the silent p23p locus |
top of page |
The mutant parasite was generated by |
Name PI/Researcher | Burda PC; Heussler VT |
Name Group/Department | Institute of Cell Biology |
Name Institute | University of Bern |
City | Bern |
Country | Switzerland |
top of page |
Name of the mutant parasite |
RMgm number | RMgm-4320 |
Principal name | PbPMP1-KO |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
top of page |
Phenotype |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Not different from wild type |
Sporozoite | Not different from wild type |
Liver stage | Not different from wild type |
Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of PMP1 and expresses mCherry under the control of the P. berghei hsp70 promoter.
Protein (function)
P. berghei Plasma Membrane Protein 1 (PbPMP1) is a 404 amino acids protein, which is conserved among Plasmodium species and contains a predicted phospholipase C/P1-S1 nuclease domain in addition to a signal peptide and a C-terminal transmembrane domain An RNAseq study, primarily of blood stage P. berghei parasites, showed highest expression of PbPMP1 in schizont stages, in comparison to rings, trophozoites, gametocytes and ookinetes18. Furthermore, PbPMP1- derived peptides were detected in a study comparing the proteome of male and female gametocytes, indicating expression at this stage.
PMP1 is located at the parasite plasma membrane (PPM) in all life cycle stages (see mutant RMgm-4319 for more details.
Phenotype
The mutant shows normal development throughout the complete life cycle
Additional information
Other mutants
RMgm-4319: a mutant expressing a C-terminal GFP-tagged version of PMP1 |