RMgmDB - Rodent Malaria genetically modified Parasites

Back to search results

Summary

RMgm-4320
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_0809400; Gene model (P.falciparum): PF3D7_0908000; Gene product: plasma membrane protein 1, putative (PMP1)
PhenotypeNo phenotype has been described
Last modified: 10 September 2017, 16:37
  *RMgm-4320
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 28851956
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone RMgm-928
Other information parent lineP.berghei ANKA 1804cl1 (RMgm-928) is a reference line expressing mCherry under the control of the P. berghei hsp70 promoter. The mutant does not contain a drug-selectable marker. The mCherry expression cassette has been introduced into the silent p23p locus
The mutant parasite was generated by
Name PI/ResearcherBurda PC; Heussler VT
Name Group/DepartmentInstitute of Cell Biology
Name InstituteUniversity of Bern
CityBern
CountrySwitzerland
Name of the mutant parasite
RMgm numberRMgm-4320
Principal namePbPMP1-KO
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of PMP1 and expresses mCherry under the control of the P. berghei hsp70 promoter.

Protein (function)
P. berghei Plasma Membrane Protein 1 (PbPMP1) is a 404 amino acids protein, which is conserved among Plasmodium species and contains a predicted phospholipase C/P1-S1 nuclease domain in addition to a signal peptide and a C-terminal transmembrane domain An RNAseq study, primarily of blood stage P. berghei parasites, showed highest expression of PbPMP1 in schizont stages, in comparison to rings, trophozoites, gametocytes and ookinetes18. Furthermore, PbPMP1- derived peptides were detected in a study comparing the proteome of male and female gametocytes, indicating expression at this stage.
PMP1 is located at the parasite plasma membrane (PPM) in all life cycle stages (see mutant RMgm-4319 for more details.

Phenotype
The mutant shows normal development throughout the complete life cycle

Additional information

Other mutants
RMgm-4319: a mutant expressing a C-terminal GFP-tagged version of PMP1


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0809400
Gene Model P. falciparum ortholog PF3D7_0908000
Gene productplasma membrane protein 1, putative
Gene product: Alternative namePMP1
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6