RMgmDB - Rodent Malaria genetically modified Parasites

Back to search results

Summary

RMgm-1224
Malaria parasiteP. yoelii
Genotype
DisruptedGene model (rodent): PY17X_0522400; Gene model (P.falciparum): Not available; Gene product: 18S ribosomal RNA (small subunit (ssu) of the c-type ribosomal rna gene unit)
PhenotypeNo phenotype has been described
Last modified: 26 March 2015, 13:19
  *RMgm-1224
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 25759501
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. yoelii
Parent strain/lineP. y. yoelii 17XNL
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherQi Y; Xin-zhuan S
Name Group/DepartmentState Key Laboratory of Cellular Stress Biology
Name InstituteInnovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University
CityXiamen, Fujian
CountryChina
Name of the mutant parasite
RMgm numberRMgm-1224
Principal nameCssu
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot tested
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of the small subunit (ssu) of the c-type ribosomal rna gene unit (located on chromosome 5).

Protein (function)
P. yoelii contains four distinct copies of the ribosomal RNA gene units (A-D), each encoding the following rRNA molecules: large subunit (LSU; 28 S) rRNA, small subunit (SSU; 18 S) rRNA and 5.8 S rRNA .
Based on differences in sequence and expression the four gene units are divided into the blood stage A-type (a- and b-unit) and S-type (c- and d-units), which is transcribed mainly in the proliferative stages in the mosquito.  The A-type ribosomes are present in the liver and blood stages of the parasite, and the S-type ribosomes are the predominant types produced during development in the mosquito.
P. yoelii gene models of the 4 SSU (18S) rRNA genes are: PY17X_0625700, PY17X_0701110, PY17X_1249340, PY17X_0522400

Phenotype
The phenotype of oocysts and sporozoites has been analysed (but not in great detail). Oocysts and salivary gland sporozoites are formed. Sporozoites are infective to mice.

Additional information
In P. berghei disruption of the c-type rRNA gene unit also does not have a clear effect on parasite development. Oocyst maturation may be slightly delayed. See RMgm-21 and RMgm-22.

Other mutants
RMgm-1223 - A P. yoelii mutant lacking expression of the small subunit (ssu) of the d-type ribosomal rna gene unit


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PY17X_0522400
Gene Model P. falciparum ortholog Not available
Gene product18S ribosomal RNA
Gene product: Alternative namesmall subunit (ssu) of the c-type ribosomal rna gene unit
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedureWR99210
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6