RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-1021
Malaria parasiteP. berghei
Genotype
DisruptedGene model (rodent): PBANKA_1437500; Gene model (P.falciparum): PF3D7_1222600; Gene product: AP2 domain transcription factor AP2-G (AP2-G; ApiAP2)
Phenotype Gametocyte/Gamete; Fertilization and ookinete;
Last modified: 22 March 2014, 19:57
  *RMgm-1021
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 24572359
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA 820cl1m1cl1 (RMgm-164)
Other information parent lineP. berghei ANKA 820cl1m1cl1 (RMgm-164) is a reference ANKA mutant line which expresses GFP under control of a male and RFP under control of a female gametocyte specific promoter. This reference line does not contain a drug-selectable marker (PubMed: PMID: 19438517).
The mutant parasite was generated by
Name PI/ResearcherSinha, A; Waters, AP
Name Group/DepartmentWellcome Trust Centre for Molecular Parasitology
Name InstituteUniversity of Glasgow
CityGlasgow
CountryUK
Name of the mutant parasite
RMgm numberRMgm-1021
Principal namepbap2-g (-)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNo gametocytes are formed
Fertilization and ookineteNo gametocytes are formed
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant lacks expression of AP2-G

Protein (function)
PbAP2-G belongs to the 27 Plasmodium ApiAP2 family of transcription factors

Phenotype
No gametocytes are formed. Evidence is presented that PbAP2-G is essential for commitment of asexually replicating forms to sexual development

Additional information

Other mutants


  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1437500
Gene Model P. falciparum ortholog PF3D7_1222600
Gene productAP2 domain transcription factor AP2-G
Gene product: Alternative nameAP2-G; ApiAP2
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedYes
Name of PlasmoGEM construct/vectorPbGEM-072446
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6